Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bong, S.M.; Son, K.B.; Yang, S.W.; Park, J.W.; Cho, J.W.; Kim, K.T.; Kim, H.; Kim, S.J.; Kim, Y.J.; Lee, B.I.
    Crystal structure of human myotubularin-related protein 1 provides insight into the structural basis of substrate specificity (2016), PLoS ONE, 11, e0152611 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.95 expressed in Escherichia coli Rosetta 2(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.95 sitting drop vapor diffusion method, using 100 mM Na-HEPES (pH 7.0) and 15% (w/v) polyethylene glycol 20000 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.95 C438S inactive Homo sapiens
3.1.3.95 D443A almost inactive Homo sapiens
3.1.3.95 K285A the mutation has little effect on the phosphatase activity compared to the wild type enzyme Homo sapiens
3.1.3.95 K484A the mutant exhibits no phosphatase activity Homo sapiens
3.1.3.95 R444A inactive Homo sapiens
3.1.3.95 R444A/R480A inactive Homo sapiens
3.1.3.95 R480A the mutant shows slightly reduced phosphatase activity compared to the wild type enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.95 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O Homo sapiens
-
 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate
-
 ?
3.1.3.95 1-phosphatidyl-1D-myo-inositol 5-phosphate + H2O Homo sapiens
-
 1-phosphatidyl-1D-myo-inositol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.95 Homo sapiens Q13613
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.95 Ni-NTA column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.95 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O
-
 Homo sapiens 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate
-
 ?
3.1.3.95 1-phosphatidyl-1D-myo-inositol 5-phosphate + H2O
-
 Homo sapiens 1-phosphatidyl-1D-myo-inositol + phosphate
-
 ?
3.1.3.95 di-C8 phosphatidylinositol 3,5-bisphosphate + H2O
-
 Homo sapiens ?
-
 ?
3.1.3.95 di-C8 phosphatidylinositol 5-phosphate + H2O
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.95 monomer dynamic light scattering Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.3.95 MTMR1
-
 Homo sapiens
3.1.3.95 myotubularin-related protein 1
-
 Homo sapiens